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| Registros recuperados : 2 | |
| 1. |  | FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. Soil and rhizosphere bacterial diversity in maize agro-ecosystem. Sustainable Agriculture Research, 2017, v.6 (3): 35-51. Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017.| Biblioteca(s): INIA Las Brujas. |
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| 2. | | FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33.| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 2 | |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
09/11/2017 |
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Actualizado : |
12/12/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Soil and rhizosphere bacterial diversity in maize agro-ecosystem. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 35-51. |
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ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
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DOI : |
10.5539/sar.v6n3p |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. |
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Contenido : |
ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial ... Presentar Todo |
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Palabras claves : |
DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668
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Marc : |
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001 1057747
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022 $a1927-050X (Print) // 1927-0518 (Online)
024 7 $a10.5539/sar.v6n3p$2DOI
100 1 $aFEDERICI, M.
245 $aSoil and rhizosphere bacterial diversity in maize agro-ecosystem.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017.
520 $aABSTRACT. Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices. Copyright © Canadian Center of Science and Education
653 $aDGGE
653 $aMETAGENOMICS
653 $aPYROSEQUENCING
653 $aRHIZOSPHERE
653 $aSOIL BACTERIA
653 $aTRANSGENIC MAIZE
700 1 $aBAJSA, N.
700 1 $aLAGURARA, P.
700 1 $aREVALE, S.
700 1 $aMARCONDES, J.A.
700 1 $aDALLA RIZZA, M.
773 $tSustainable Agriculture Research, 2017$gv.6 (3): 35-51.
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